Centrifuges for inactivated vaccines

The main purpose of centrifugation in the production of inactivated vaccines

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  Cell culture fluid clarification

  Remove impurities such as cell debris and culture medium components in the culture medium to obtain pure culture supernatant containing antigens.
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  Virus or bacteria separation

  Separate the virus or bacteria in the culture supernatant from the culture medium components to increase the antigen concentration.
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  Impurity removal

  Remove impurities such as host cell proteins and nucleic acids in the antigen solution to improve the purity of the vaccine.
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  Adjuvant mixing

  In the later stage of vaccine preparation, the antigen can be mixed with the adjuvant and homogenized by centrifugation.
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  Disc separator

  Large processing capacity, continuous operation, suitable for large-scale production, often used for cell culture fluid clarification and virus concentration.

Centrifugation process in inactivated vaccine production

Cell culture: Cultivate pathogens in a suitable culture medium to make them proliferate in large quantities.
Cell harvesting: Separate cells from the culture medium by centrifugation or filtration.
Cell lysis: Destroy cells by physical or chemical methods to release antigens.
Clarification: Use a centrifuge to remove large particle impurities such as cell fragments and unlysed cells.
Concentration: Concentrate antigens by centrifugation to increase their content.
Purification: Use different centrifugation conditions and media to remove impurities such as proteins and nucleic acids.
Inactivation: Physically or chemically inactivate antigens.
Adjuvant addition: Mix inactivated antigens with adjuvants to enhance the immune effect.
Filling: Fill the prepared vaccine into a container.

Factors affecting centrifugal effect

Centrifugal force: The magnitude of the centrifugal force directly affects the separation effect. The higher the speed, the greater the centrifugal force.
Centrifugation time: The longer the centrifugation time, the better the separation effect, but too long centrifugation time may cause the sample to overheat or denature.
Temperature: Temperature has a greater impact on biological samples, and the temperature needs to be controlled according to the properties of the sample.
Medium density: The density of the medium affects the sedimentation rate of the particles.
Centrifuge tube material: The material of the centrifuge tube should have good corrosion resistance and biocompatibility.

Precautions

Aseptic operation: The entire centrifugation process should be carried out under sterile conditions to prevent contamination.
Equipment maintenance: Clean and maintain the centrifuge equipment regularly to ensure its stable performance.
Process optimization: Optimize the centrifugation process according to different vaccine types and production scales.