Fermentation Broth Treatment

In the field of industrial biotechnology, the efficiency of downstream processing is determined by the precision of primary separation. Fermentation broths are complex systems containing shear-sensitive microorganisms, metabolic by-products, and residual media. Huading Separator offers specialized disc centrifuges designed to handle these challenges with maximum biomass yield and purity.

━ Applications

Bacteria and Yeast Harvesting: E. coli, lactic acid bacteria, and yeast cultures.

Mycelia Separation: Specialized designs for fibrous fermentation broths.

Enzyme Recovery: Rapid separation to prevent enzymatic degradation.

Amino Acids & Organic Acids: High-throughput clarification for large-scale production.

━ Process Steps

Pre-treatment: Adjust pH and temperature of the broth to optimize viscosity and particle stability.

Feeding: The broth is pumped into the disc stack centrifuge through a gentle feeding system to minimize shear stress on cells.

Separation: Under high centrifugal force, microorganisms are concentrated at the periphery, while the clarified liquid moves to the center.

Discharge: Concentrated biomass is intermittently discharged via the self-cleaning bowl, while the clear phase is discharged under pressure.

CIP Cleaning: Automated Cleaning-in-Place cycles ensure no biological residue remains between batches.

━ Key Considerations

Shear Sensitivity: Some cells are fragile; ensure the centrifuge speed and inlet design are optimized to prevent cell lysis.

Temperature Control: Fermentation products are often heat-sensitive; cooling jackets may be required to maintain biological activity.

Aseptic Integrity: High-quality mechanical seals are essential to prevent environmental contamination.